BIOMEDICAL ARTICLES
Two-dimensional blue native polyacrylamide gel electrophoresis.
Multiprotein complexes play crucial roles in nearly all cell biological processes. Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) is a powerful method to study these complexes. It is a native protein separation method that relies on the dye Coomassie blue to confer negative charge for separation. It has […]
Isolation of amyloplasts.
Two different methods for the preparation of starch-rich plastids are described together with protocols for the determination of plastid yield, purity, and intactness. The preparation of amyloplasts from maize endosperm and oilseed rape embryos are given as examples, but the protocols could be adapted for the isolation of starch-rich plastids […]
Photoactivation and imaging of photoactivatable fluorescent proteins.
A major advance in the microscopic study of cells and tissues is the introduction of photoactivatable fluorescent proteins, which can specifically mark proteins of interest within a living cell. Fluorescent proteins are now available that allow a pool of molecules to be \”turned on\” by photoactivation. […]
Rho GTPase activation assays.
The Rho GTPase family of signaling proteins controls a wide range of highly dynamic cellular processes. Activation of Rho GTPases can be investigated and quantified in cell extracts using so-called pull-down assays. Proteins that bind specifically to the activated form of the Rho GTPase are used to capture it […]
Evaluation of a protein microarray method for immuno-typing erythrocytes in whole blood.
All donor blood samples must be tested pre-transfusion to determine the blood type of donor erythrocytes, based on the ABO typing system. Current methods of testing are well characterised, but require a number of processing steps prior to analysis. In addition, […]
Production of novel anti-recombinant human erythropoietin monoclonal antibodies and development of a sensitive enzyme-linked immunosorbent assay for detection of bioactive human erythropoietin.
Erythropoietin (EPO) is a growth factor, regulating the proliferation and differentiation of erythroid progenitor cells. In this study, we generated five monoclonal antibodies (mAbs) that reacted specifically with recombinant human EPO […]
Development and validation of an ELISA method for detection of growth arrest specific 6 (GAS6) protein in human plasma.
Gas6 protein is possibly involved in human diseases, but a validated plasma assay is lacking. So, we developed a sandwich enzyme-linked immunosorbent assay (ELISA) method using commercially available reagents. An appropriate plasma-based matrix was […]
ANA detected by ELISA using nucleus of egg cell as antigen.
Antinuclear antibodies, ANA, were usually detected with antigen of somatic cell nucleus. It has not been reported to detect ANA with egg cell nucleus as antigen. Enzyme linked immuosorbent assay, ELISA, coated with yolk was developed to detect ANA in our laboratory. […]
Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase antibodies for universal detection.
The biosynthesis of steroids from steroidogenic cells are catalyzed by the two major enzymes, P450 side-chain cleavage enzyme (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD). This article describes the construction of two novel polyclonal antibodies against conserved recombinant protein […]
Serological diagnosis of syphilis: enzyme-linked immunosorbent assay to measure antibodies to individual recombinant Treponema pallidum antigens.
We standardized an indirect ELISA for measurement of serum antibody levels to four individual treponemal recombinant proteins that have been commonly used in a number of commercial EIAs, mostly as a mixture of antigens. When tested with […]
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