Generation and utilization of P450 cholesterol side-chain cleavage enzyme and 3beta-hydroxysteroid dehydrogenase antibodies for universal detection.
The biosynthesis of steroids from steroidogenic cells are catalyzed by the two major enzymes, P450 side-chain cleavage enzyme (P450scc) and 3beta-hydroxysteroid dehydrogenase (3beta-HSD). This article describes the construction of two novel polyclonal antibodies against conserved recombinant protein […]

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Serological diagnosis of syphilis: enzyme-linked immunosorbent assay to measure antibodies to individual recombinant Treponema pallidum antigens.
We standardized an indirect ELISA for measurement of serum antibody levels to four individual treponemal recombinant proteins that have been commonly used in a number of commercial EIAs, mostly as a mixture of antigens. When tested with […]

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Development of a storage-compatible microtiter plate-based technique for lymphocyte proliferation.
The lymphocyte proliferation assay (LPA) is an important tier 1 test to assess non-specific lymphocyte function, in vitro. However, this assay requires fresh preparation, is time consuming, and labor intensive. Developing a plate coating technique for lymphocyte proliferation that is both stable and […]

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Development of rapid and sensitive one-step direct enzyme linked immunosorbent assay for 17-alpha-OH-progesterone in serum.
Using a homologous combination of immunogen and enzyme conjugate, a highly specific and sensitive Enzyme Linked Immunosorbent Assay (ELISA) was developed to measure 17-alpha-hydroxy-progesterone (17-alpha-OH-P) in human serum. The antiserum was raised against 17-alpha-hydroxy-progesterone-3-O-carboxymethyloxime bovine serum albumin (17-alpha-OH-P-3-O-CMO-BSA) […]

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Family history and colorectal cancer survival in women.
Purpose Family history of colorectal cancer may be a phenotype for numerous genetic mutations which increase colorectal cancer risk and may affect survival after diagnosis. We examined the relationship between self-reported first-degree family history of colorectal cancer and survival. Methods We identified female Wisconsin residents […]

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Usefulness of preoperative multidetector-row computed tomography in evaluating the extent of invasive lobular carcinoma in patients with or without neoadjuvant chemotherapy.
BACKGROUND: The present study was conducted to assess the clinical usefulness of multidetector-row CT (MDCT) in determining the extent of invasive lobular carcinoma (ILC) and especially the extent of residual tumor after […]

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Current state and future of diagnostic breast imaging.
Tsunoda-Shimizu H.
Department of Radiology, St Luke’s International Hospital, Tokyo, Japan, hirokots@luke.or.jp.

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Varicella-zoster virus hepatitis in polymyositis.
A 31-year-old woman had recurrent mild flare-ups of polymyositis for years. Fourteen days after low-dose methotrexate was added in an attempt to taper the corticosteroid, she began to feel abdominal and lower back pain, followed by generalized pustulosis, severe liver dysfunction, and disseminated intravascular coagulation. On the diagnosis […]

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F-18 Fluoride Positron Emission Tomography of the Hip for Osteonecrosis.
Osteonecrosis (ON) of the femoral head continues to be a devastating disorder for young patients. We evaluated the F-18 fluoride positron emission tomography (PET) imaging modality for use in detection of the bone involved in ON of the hip. We retrospectively reviewed the […]

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Bioactive glass induced in vitro apatite formation on composite GBR membranes.
The aim of this study was to investigate in vitro bioactivity of different thermoplastic biodegradable barrier membranes. Three experimental GBR membranes were fabricated using Poly(epsilon-caprolactone-co-D: ,L: -lactide) P(CL/DL-LA) and particulate bioactive glass S53P4 (BAG; granule size 90-315 mum): (A) composite membrane with […]

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